Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P33991
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
CDC21 homolog, DNA replication licensing factor MCM4, P1-CDC21
Specificity Sensitivity:
MCM4 (Ab-54) antibody detects endogenous levels of total MCM4 protein.
References:
Musahl C., Eur. J. Biochem. 230:1096-1101(1995).
Livingston R.J., Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human testis tissue using MCM4 (Ab-54) antibody.
Western blot analysis of extracts from HepG2 cells, using MCM4 (Ab-54) antibody.