Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human DDX3/DEAD-box Protein 3 around the phosphorylation site of threonine 322 (V-A-TP-P-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O00571
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
D1PAS1 related sequence 2, D1PAS1-RS2, DBX, DDX3X, DEAD-box RNA helicase DEAD3, DEAD-box protein 3, DEAD-box, X isoform, DEAD3, EC 3.6.1.-, ERH, Embryonic RNA helicase, HLP2, Helicase-like protein 2
Specificity Sensitivity:
DDX3/DEAD-box Protein 3 (Ab-322) antibody detects endogenous levels of total DDX3/DEAD-box Protein 3 protein.
References:
Chung J., Korean J. Biochem. 27:193-197(1995).
Owsianka A.M., Virology 257:330-340(1999).
Lahn B.T., Science 278:675-680(1997).
Gevaert K., Proteomics 5:3589-3599(2005).
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using DDX3/DEAD-box Protein 3 (Ab-322) antibody.
Western blot analysis of extracts from HepG2 cells, using DDX3/DEAD-box Protein 3 (Ab-322) antibody.