Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CRYAB/Crystallin-α-B around the phosphorylation site of serine 59 (A-P-SP-W-F).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
CRYAB/Crystallin-α-B (Ab-59) antibody detects endogenous levels of total CRYAB/Crystallin-α-B protein.
References:
Dubin R.A., Genomics 7:594-601(1990).
Iwaki A., Neurosci. Lett. 140:89-92(1992).
Kovalyov L.I., Electrophoresis 16:1160-1169(1995).
Scanlan M.J., Int. J. Cancer 83:456-464(1999).
Western blot analysis of extracts from K562 cells, treated with Ca2+ (40nM, 30mins), using CRYAB/Crystallin-α-B (Ab-59) antibody.