Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human c-Jun around the phosphorylation site of serine 249 (M-E-SP-Q-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05412
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
AH119, AP-1, AP1, Activator protein 1, Jun A, Proto-oncogene c-jun, RJG-9, V-jun avian sarcoma virus 17 oncogene homolog, c-Jun, p39
Specificity Sensitivity:
c-Jun (Ab-249) antibody detects endogenous levels of total c-Jun protein.
References:
Thomas Herdegen, J. Neurosci., Jul 1998; 18: 5124
Toru Kurokawa,Invest. Ophthalmol. Vis. Sci., Nov 1999; 40: 3006.
T Kameda, Cell Growth Differ., May 1997; 8: 495.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using c-Jun (Ab-249) antibody.
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using c-Jun (Ab-249) antibody.