• Catalog Number:

  E1A0855B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human CDC25B around the phosphorylation site of serine 353 (R-R-SP-V-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P30305

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  CDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2

• Specificity Sensitivity:

  CDC25B (Ab-353) antibody detects endogenous levels of total CDC25B protein.

• References:

  Sanae Uchida, J. Cell Sci., Jun 2004; 117: 3011 - 3020. Christina Karlsson, J. Cell Biol., Aug 1999; 146: 573. Arne Lindqvist, J. Cell Biol., Oct 2005; 171: 35 - 45.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC25B (Ab-353) antibody. Western blot analysis of extracts from Raw264.7 cells, treated with UV (15mins), using CDC25B (Ab-353) antibody.

Peptide - + Immunofluorescence analysis of HeLa cells, using CDC25B (Ab-353) antibody.