• Catalog Number:

  E1A0850B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human CD50/ICAM-3 around the phosphorylation site of serine 518 (S-G-SP-Y-H).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P32942

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  CD50 antigen, CDw50, ICA3, ICAM-R, ICAM3, Intercellular adhesion molecule-3 precursor

• Specificity Sensitivity:

  CD50/ICAM-3 (Ab-518) antibody detects endogenous levels of total CD50/ICAM-3 protein.

• References:

  Fawcett J., Nature 360:481-484(1992). de Fougerolles A.R., J. Exp. Med. 177:1187-1192(1993). Funatsu O., Eur. J. Biochem. 268:1020-1029(2001). Liu T., J. Proteome Res. 4:2070-2080(2005).

Peptide - + Western blot analysis of extracts from NIH/3T3 cells, treated with TNF-a (20ng/ml, 5mins), using CD50/ICAM-3 (Ab-518) antibody. Immunofluorescence analysis of A549 cells, using CD50/ICAM-3 (Ab-518) antibody.