Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Caspase 9 around the phosphorylation site of tyrosine153 (L-A-YP-I-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Caspase 9 (Ab-153) antibody detects endogenous levels of total Caspase 9 protein.
References:
Yuanming Hu, J. Biol. Chem., Dec 1998; 273: 33489.
Michael H. Cardone, Science, Nov 1998; 282: 1318.
C.S. Thota, Biol Reprod, Dec 2005; 73: 1191 - 1198.
Peptide - +
Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using Caspase 9 (Ab-153) antibody.
Immunofluorescence analysis of HeLa cells, using Caspase 9 (Ab-153) antibody.