Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CaMK1-α around the phosphorylation site of threonine 177 (L-S-TP-A-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q14012
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
CAM kinase I, CAMK1, Calcium/calmodulin-dependent protein kinase type I, EC 2.7.11.17, KCC1, KCC1A, kinase CaMK1-alpha
Specificity Sensitivity:
CaMK1-α (Ab-177) antibody detects endogenous levels of total CaMK1-α protein.
References:
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Hsu L.-S., J. Biol. Chem. 276:31113-31123(2001).
Qin H., J. Biol. Chem. 278:48570-48579(2003).
Western blot analysis of extracts from COLO cells, using CaMK1-α (Ab-177) antibody.