• Catalog Number:

  E1A0817B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of tyrosine 326 (N-D-YP-G-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  AKT, AKT1 kinase, C-AKT, EC 2.7.11.1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1

• Specificity Sensitivity:

  Akt (Ab-326) antibody detects endogenous levels of total Akt protein.

• References:

  Amy D. Sinor, J. Neurosci., Sep 2004; 24: 8531 - 8541. Daniela Matei, Clin. Cancer Res., Jan 2004; 10: 681. Jesika Faridi, Clin. Cancer Res., Aug 2003; 9: 2933.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-326) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using Akt (Ab-326) antibody.

Peptide - + Immunofluorescence analysis of HeLa cells, using Akt (Ab-326) antibody.