Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Rb around the phosphorylation site of serine 811 (L-K-SP-P-Y).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P06400
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Other Names:
P105-RB, PP105, PP110, RB-1, RB1, Retinoblastoma-associated protein
Specificity Sensitivity:
Rb (Ab-811) antibody detects endogenous levels of total Rb protein.
References:
Hogg A., Oncogene 7:1445-1451(1992).
Dunham A., Nature 428:522-528(2004).
The MGC Project Team, me Res. 14:2121-2127(2004).
Peptide - +
Western blot analysis of extracts from HeLa cells, treated with Calyculin A (50nM, 30mins), using Rb (Ab-811) antibody.
Immunofluorescence analysis of HuvEc cells, using Rb (Ab-811) antibody.