Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PKD2 around the phosphorylation site of serine 876 (R-I-SP-V-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q9BZL6
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
EC 2.7.11.13, HSPC187, KPCD2, KPCO, PRKD2, Protein kinase C, D2 type, Protein kinase D2, Protein kinase D2 homolog, kinase PKD2, nPKC-D2
Specificity Sensitivity:
PKD2 (Ab-876) antibody detects endogenous levels of total PKD2 protein.
References:
Nicholas Obermüller, Am J Physiol Renal Physiol, Dec 1999; 277: 914.
Rong Ma, Mol. Cell. Biol., Sep 2005; 25: 8285 - 8298.
Dana R. Rundle, J. Biol. Chem., Jul 2004; 279: 29728 - 29739.
Sabine Sturany, J. Biol. Chem., Aug 2002; 277: 29431 - 29436.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PKD2 (Ab-876) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), using PKD2 (Ab-876) antibody.