Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PKC α around the phosphorylation site of tyrosine 657 (F-S-YP-V-N).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P17252
Applications:
WB: 1:500~1:1000 ELISA: 1:5000
Other Names:
EC 2.7.11.13; kinase PKC-alpha; KPCA; PKC III; PKC-A; PKC-alpha; PKC-III; PRKACA; PRKCA; Protein kinase C, alpha type
Specificity Sensitivity:
PKC α (Ab-657) antibody detects endogenous levels of total PKC α protein.
References:
Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143(1991).
Western blot analysis of extracts from MCF-7 cells and Jurkat cells, using PKC α (Ab-657) antibody.