Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 383 (M-R-TP-L-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Chk2 (Ab-383) antibody detects endogenous levels of total Chk2 protein.
References:
Mark Katory, AACR Meeting Abstracts, Mar 2004; 2004: 838.
Shannon L. Gibson, Cancer Res., Dec 2005; 65: 10734 - 10741.
Jinwoo Ahn, J. Biol. Chem., Dec 2002; 277: 48418 - 48426.
Nabil H. Chehab, Genes & Dev., Feb 2000; 14: 278.
Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Chk2 (Ab-383) antibody.