• Catalog Number:

  E1A0774B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 69 (A-R-TP-S-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10415

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  Apoptosis regulator Bcl-2, BCL2

• Specificity Sensitivity:

  BCL-2 (Ab-69) antibody detects endogenous levels of total BCL-2 protein.

• References:

  H. Kurosawa, Am J Physiol Gastrointest Liver Physiol, Jun 1997; 272: 1587. JR Park, Blood, Aug 1995; 86: 868 - 876. A Plettenberg, Am. J. Pathol., Mar 1995; 146: 651. JM Ben-Ezra, Am. J. Pathol., Nov 1994; 145: 1036.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using BCL-2 (Ab-69) antibody. Western blot analysis of extracts from K562 cells, using BCL-2 (Ab-69) antibody.

Peptide - + Immunofluorescence analysis of HepG2 cells, using BCL-2 (Ab-69) antibody.