Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK β1 around the phosphorylation site of serine 181 (S-S-SP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
AMPK β1 (Ab-181) antibody detects endogenous levels of total AMPK β1 protein.
References:
Carling D., Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Stapleton D., FEBS Lett. 409:452-456(1997).
Yamagata K., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using AMPK β1 (Ab-181) antibody.
Western blot analysis of extracts from K562 cells, using AMPK β1 (Ab-181) antibody.