• Catalog Number:

  E1A0762B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Actin-pan (α/β/γ) around the phosphorylation site of tyrosine 53/55 (D-S-YP-V-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P60709/P63261

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1; ACTB, Actin, cytoplasmic 1, Beta-actin; ACTG, ACTG1, actin, cytoplasmic 2, gamma-actin

• Specificity Sensitivity:

  Actin-pan (α/β/γ) (Ab-53/55) antibody detects endogenous levels of total Actin-pan (α/β/γ) protein.

• References:

  A. J. MacQueen, Mol. Biol. Cell, Jul 2005; 16: 3247 - 3259. Isaac Rabinovitz, J. Cell Biol., Dec 1997; 139: 1873. Deanne H. Hryciw, Am J Physiol Renal Physiol, Jun 2005; 288: F1227 - F1235. Derek T. Warren, J. Cell Sci., Apr 2002; 115: 1703.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Actin-pan (α/β/γ) (Ab-53/55) antibody. Western blot analysis of extracts from mouse brain cells, using Actin-pan (α/β/γ) (Ab-53/55) antibody.

Peptide - + Immunofluorescence analysis of HuvEc cells, using Actin-pan (α/β/γ) (Ab-53/55) antibody.