Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human 53BP1 around the phosphorylation site of serine 6 (T-G-SP-Q-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q12888
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Other Names:
P531, TP53B, TP53BP1, TRP53BP1, Tumor suppressor p53-binding protein 1, p53-binding protein 1
Specificity Sensitivity:
53BP1 (Ab-6) antibody detects endogenous levels of total 53BP1 protein.
References:
Iwabuchi K., J. Biol. Chem. 273:26061-26068(1998).
Iwabuchi K., Proc. Natl. Acad. Sci. U.S.A. 91:6098-6102(1994).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Peptide - +
Western blot analysis of extracts from A549 cells, using 53BP1 (Ab-6) antibody.
Immunofluorescence analysis of HeLa cells, using 53BP1 (Ab-6) antibody.