Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human 14-3-3 θ/τ around the phosphorylation site of serine 232 (S-D-SP-A-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P27348
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000
Other Names:
14-3-3 protein T-cell, 14-3-3 protein tau, 1433T, 143T, HS1 protein, YWHAQ
Specificity Sensitivity:
14-3-3 θ/τ (Ab-232) antibody detects endogenous levels of total 14-3-3 θ/τ protein.
References:
Nielsen P.J., im. Biophys. Acta 1088:425-428(1991).
Leffers H., J. Mol. Biol. 231:982-998(1993).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Peptide - +
Western blot analysis of extracts from HeLa cells, using 14-3-3 θ/τ (Ab-232) antibody.
Immunofluorescence analysis of HeLa cells, using 14-3-3 θ/τ (Ab-232) antibody.