Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human YAP around the phosphorylation site of serine 127 (A-H-SP-S-P).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P46937
Applications:
WB: 1:500~1:1000 ELISA: 1:1000
Other Names:
65 kDa Yes-associated protein, YAP1, YAP65
Specificity Sensitivity:
YAP (Ab-127) antibody detects endogenous levels of total YAP protein.
References:
Rami I. Aqeilan, Cancer Res., Aug 2005; 65: 6764 - 6772.
Michael Howell, J. Biol. Chem., Jun 2004; 279: 26300 - 26306.
Akihiko Komuro, J. Biol. Chem., Aug 2003; 278: 33334 - 33341.
Masataka Hoshino, J. Cell Biol., Feb 2006; 172: 589 - 604.
Western blot analysis of extracts from HepG2 cells, treated with Wortmannin (40nM, 24hours), using YAP (Ab-127) antibody.