Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PKC θ around the phosphorylation site of threonine 538(T-N-TP-F-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
PKC θ (Ab-538) antibody detects endogenous levels of total PKC θ protein.
References:
Chang J.D., J. Biol. Chem. 268:14208-14214(1993).
Chang J.D., J. Biol. Chem. 269:31322-31322(1994).
Baier G., J. Biol. Chem. 268:4997-5004(1993).
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PKC θ (Ab-538) antibody.
Western blot analysis of extracts from 293 cells, using PKC θ (Ab-538) antibody.