Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PKC α/β2 around the phosphorylation site of threonine 638 (V-L-TP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P17252
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
EC 2.7.11.13, KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha
Specificity Sensitivity:
PKC α/β2 (Ab-638) antibody detects endogenous levels of total PKC α/β2 protein.
References:
Maria Nikodemova, J. Biol. Chem., May 2007; 282: 15208 - 15216.
Pedro J. Cejas, J. Biol. Chem., Aug 2005; 280: 28412 - 28423.
Dan Chen, J. Biol. Chem., Feb 2004; 279: 4829 - 4839.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC α/β2 (Ab-638) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with UV (15mins), using PKC α/β2 (Ab-638) antibody.