Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PBK/TOPK around the phosphorylation site of threonine 9 (F-K-TP-P-S).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q96KB5
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:000
Other Names:
EC 2.7.12.2, MPAKK-like protein kinase cap site form 2, NORI-3, TOPK
Specificity Sensitivity:
PBK/TOPK (Ab-9) antibody detects endogenous levels of total PBK/TOPK protein.
References:
J. D. Dougherty, J. Neurosci., Nov 2005; 25: 10773 - 10785.
Asit K. Nandi, Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 4287.
Bulent Ozpolat, AACR Meeting Abstracts, Apr 2006; 2006: 621.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PBK/TOPK (Ab-9) antibody.
Western blot analysis of extracts from COS7 cells, treated with Nocodazole (1ug/ml, 16hours), using PBK/TOPK (Ab-9) antibody.