Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PAK4/5/6 around the phosphorylation site of serine 474 (R-K-SP-L-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
O96013
Applications:
WB: 1:500~1:1000 ELISA: 1:5000
Other Names:
EC 2.7.11.1, PAK 4, PAK-4, kinase PAK4, p21-activated kinase 4
Specificity Sensitivity:
PAK4/5/6 (Ab-474) antibody detects endogenous levels of total PAK4/5/6 protein.
References:
Abo A., EMBO J. 17:6527-6540(1998).
Melnick M.B.; Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
Hirosawa M., DNA Res. 6:329-336(1999).
Western blot analysis of extracts from K562 cells, treated with PMA (125ng/ml, 30mins), using PAK4/5/6 (Ab-474) antibody.