Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human NPM around the phosphorylation site of threonine 199 (R-D-TP-P-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
NPM (Ab-199) antibody detects endogenous levels of total NPM protein.
References:
Philipp B Staber, Blood, Aug 2007; 10.1182/blood-2007-02-071258.
Brunangelo Falini, Blood, Nov 1999; 94: 3509 - 3515.
Jean-François Honorat, Blood, May 2006; 107: 4130 - 4138.
Kotaro Funato, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 291.
Peptide - +
Western blot analysis of extracts from Jurkat cells, using NPM (Ab-199) antibody.
Immunofluorescence analysis of HepG2 cells, using NPM (Ab-199) antibody.