Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Mst1/Mst2 around the phosphorylation site of threonine183 (R-N-TP-V-I).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Mst1/2 (Ab-183) antibody detects endogenous levels of total Mst1/Mst2 protein.
References:
Patricia M. De Souza, Blood, May 2002; 99: 3432 - 3438.
Eliana B. Gómez, Mol. Cell. Biol., Oct 2005; 25: 8887 - 8903.
Helmut Glantschnig, J. Biol. Chem., Nov 2002; 277: 42987 - 42996.
Luigi Aurisicchio, J. Biol. Chem., Jan 1998; 273: 1477.
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Mst1/2 (Ab-183) antibody.