Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MSK1 around the phosphorylation site of serine 360(T-Y-SP-P-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
O75582
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
90 kDa ribosomal protein S6 kinase, , polypeptide 5, EC 2.7.11.1, KS6A5, MSPK1, Nuclear mitogen- and stress-activated protein kinase-1, RLPK, RPS6KA5, kinase MSK1, ribosomal protein S6 kinase, polypeptide V
Specificity Sensitivity:
MSK1 (Ab-360) antibody detects endogenous levels of total MSK1 protein.
References:
Giselle R. Wiggin, Mol. Cell. Biol., Apr 2002; 22: 2871 - 2881.
Greg Q. Butcher, J. Neurosci., Jun 2005; 25: 5305 - 5313.
Jason A. Gustin, Am J Physiol Cell Physiol, Mar 2004; 286: C547 - C555.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using MSK1 (Ab-360) antibody.
Western blot analysis of extracts from COS7 cells, treated with Sorbitol (0.4M, 30mins), using MSK1 (Ab-360) antibody.