• Catalog Number:

  E1A0681B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 286 (A-E-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02750

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Dual specificity mitogen-activated protein kinase kinase 1, EC 2.7.12.2, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1

• Specificity Sensitivity:

  MEK1 (Ab-286) antibody detects endogenous levels of total MEK1 protein.

• References:

  Shunichi Murakami, Genes & Dev., Feb 2004; 18: 290. Ronald A. Rimerman, J. Biol. Chem., May 2000; 275: 14736. Anne C. Marinovic, J. Biol. Chem., May 2002; 277: 16673 - 16681. Rohit Lal, AACR Meeting Abstracts, Apr 2006; 2006: 687.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Ab-286) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 5mins), using MEK1 (Ab-286) antibody.