Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of threonine 222 (L-T-TP-P-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
EC 2.7.11.1; kinase MAPKAPK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAPK-2; MAPKAPK2; RPS6KC1
Specificity Sensitivity:
MAPKAPK2 (Ab-222) antibody detects endogenous levels of total MAPKAPK2 protein.
References:
Zu Y.-L., Biochem. Biophys. Res. Commun. 200:1118-1124(1994).
Stokoe D., Biochem. J. 296:843-849(1993).
Coxon P.Y., Cell. Signal. 15:993-1001(2003).
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human heart tissue using MAPKAPK2 (Ab-222) antibody.
Western blot analysis of extracts from HUVEC cells, treated with Adriamycin (0.5uM, 3hours), using MAPKAPK2 (Ab-222) antibody.