• Catalog Number:

  E1A0641B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%);

• SwissProtNo:

  P23588

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  eIF-4B; Eukaryotic translation initiation factor 4B; IF4B

• Specificity Sensitivity:

  eIF4B (Ab-422) antibody detects endogenous levels of total eIF4B protein.

• References:

  Milburn S.C., EMBO J. 9:2783-2790(1990). Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Suzuki Y., Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using eIF4B (Ab-422) antibody. Western blot analysis of extracts from HepG2 cells and HUVEC cells, using eIF4B (Ab-422) antibody.

Peptide - + Immunofluorescence analysis of A549 cells, using eIF4B (Ab-422) antibody.