Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Doublecortin around the phosphorylation site of serine297 (A-K-SP-P-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O43602
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
DBCN, DCX, Doublin, LISX, Lis-X, Lissencephalin-X
Specificity Sensitivity:
Doublecortin (Ab-297) antibody detects endogenous levels of total Doublecortin protein.
References:
Des Portes V., Cell 92:51-61(1998).
Ross M.T., Nature 434:325-337(2005).
Olsen J.V., Cell 127:635-648(2006).
Kim M.H., Nat. Struct. Biol. 10:324-333(2003).
Western blot analysis of extracts from RAW264.7cells, using Doublecortin (Ab-297) antibody.