Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human ATP-Citrate Lyase around the phosphorylation site of serine 454 (T-A-SP-F-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ATP-Citrate Lyase (Ab-454) antibody detects endogenous levels of total ATP-Citrate Lyase protein.
References:
Elshourbagy N.A., Eur. J. Biochem. 204:491-499(1992).
Lord K.A., Protein Expr. Purif. 9:133-141(1997).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Western blot analysis of extracts from COS7 cells, treated with Calyculin (50nM, 30mins), using ATP-Citrate Lyase (Ab-454) antibody.