Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human WASP around the phosphorylation site of tyrosine 290 (L-I-YP-D-F).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P42768
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
IMD2, WAS, Wiskott-Aldrich syndrome protein, Wiskott-Aldrich syndrome protein homolog
Specificity Sensitivity:
WASP (Ab-290) antibody detects endogenous levels of total WASP protein.
References:
Hervé Falet, Blood, Aug 2002; 100: 2113 - 2122.
E Remold-O'Donnell, J. Immunol., May 1997; 158: 4021.
Ornella Parolini, Blood, Jul 1997; 90: 70 - 75.
Anna Shcherbina, J. Immunol., Dec 1999; 163: 6314.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using WASP (Ab-290) antibody.
Western blot analysis of extracts from HepG2 cells, using WASP (Ab-290) antibody.