Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human TNNI3 around the phosphorylation site of threonine 143 (R-P-TP-L-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P19429
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Other Names:
TNNC1, troponin I, cardiac muscle
Specificity Sensitivity:
TNNI3 (Ab-142) antibody detects endogenous levels of total TNNI3 protein.
References:
Raffaella Di Lisi, J. Biol. Chem., Sep 1998; 273: 25371.
Emile Missov, Circulation, Nov 1997; 96: 2953 - 2958.
Brandon J. Biesiadecki, J. Biol. Chem., Apr 2004; 279: 13825 – 13832.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using TNNI3 (Ab-142) antibody.
Western blot analysis of extracts from mouse heart cells, using TNNI3 (Ab-142) antibody.