Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PLK1 around the phosphorylation site of threonine210 (K-K-TP-L-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
PLK1 (Ab-210) antibody detects endogenous levels of total PLK1 protein.
References:
Xiaoqi Liu, Mol. Cell. Biol., Mar 2006; 26: 2093 - 2108.
Ranjaka W. Gunawardena, J. Biol. Chem., Jul 2004; 279: 29278 - 29285.
Anja Hanisch, Mol. Biol. Cell, Jan 2006; 17: 448 - 459.
Peptide - +
Western blot analysis of extracts from LOVO cells, treated with PMA (125ng/ml, 30mins), using PLK1 (Ab-210) antibody.
Immunofluorescence analysis of A549 cells, using PLK1 (Ab-210) antibody.