Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PLCG2 around the phosphorylation site of tyrosine 1217 (F-L-YP-D-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
PLCG2 (Ab-1217) antibody detects endogenous levels of total PLCG2 protein.
References:
Anouk Caraux, Blood, Feb 2006; 107: 994 - 1002.
Yeun Ju Kim, Mol. Cell. Biol., Nov 2004; 24: 9986 - 9999.
Jill C. Todt,J. Leukoc. Biol., Apr 2004; 75: 705 - 713.
Marie-Pierre Gratacap, J. Biol. Chem., Sep 1998; 273: 24314.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PLCG2 (Ab-1217) antibody.
Western blot analysis of extracts from RAW264.7 cells, treated with PMA (125ng/ml, 30mins), using PLCG2 (Ab-1217) antibody.