Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190/53 (R-E-SP-F-L).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
PDE4D (Ab-190/53) antibody detects endogenous levels of total PDE4D protein.
References:
Simon J. MacKenzie, J. Biol. Chem., May 2000; 275: 16609.
CÉLINE MÉHATS, FASEB J, Oct 2003; 17: 1831.
Guénaëlle Levallet, Biol Reprod, May 2007; 76: 794 - 803.
Daniel Peter, J. Immunol., Apr 2007; 178: 4820 - 4831.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PDE4D (Ab-190/53) antibody.
Western blot analysis of extracts from K562 cells, treated with H2O2 (100uM, 30mins), using PDE4D (Ab-190/53) antibody.