Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PARKIN around the phosphorylation site of serine 131 (K-D-SP-P-P).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
O60260
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
EC 6.3.2.-, PARK2, PRKN, PRKN2, Parkinson disease protein 2, Parkinson juvenile disease protein 2, ubiquitin E3 ligase PRKN
Specificity Sensitivity:
Parkin (Ab-131) antibody detects endogenous levels of total PARKIN protein.
References:
J. Menéndez, Hum. Mol. Genet., Jul 2006; 15: 2045 - 2058.
Miratul M.K. Muqit, Hum. Mol. Genet., Jan 2004; 13: 117 - 135.
Kenny K. K. Chung, Sci. Aging Knowl. Environ., Apr 2004; 2004: or11.
Western blot analysis of extracts from HeLa cells and HUVEC cells, using Parkin (Ab-131) antibody.