• Catalog Number:

  E1A0529B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 20 (T-F-SP-D-L).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

• Specificity Sensitivity:

  p53 (Ab-20) antibody detects endogenous levels of total p53 protein.

• References:

  Lloyd M. Alderson, Cancer Res., Mar 1995; 55: 999 - 1001. WE Mercer, PNAS, Aug 1990; 87: 6166. Timothy J. Underwood, AACR Meeting Abstracts, Apr 2006; 2006: 1167.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p53 (Ab-20) antibody. Western blot analysis of extracts from COS7 cells, treated with UV (5mins), using p53 (Ab-20) antibody.

Peptide - + Immunofluorescence analysis of HeLa cells, using p53 (Ab-20) antibody.