• Catalog Number:

  E1A0527B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human n-NOS around the phosphorylation site of serine 852 (F-N-SP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P29476

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  BNOS, Constitutive NOS, EC 1.14.13.39, N-NOS, NC-NOS, NOS, type I, NOS1, Neuronal NOS, Nitric-oxide synthase, brain, bNOS

• Specificity Sensitivity:

  n-NOS (Ab-852) antibody detects endogenous levels of total n-NOS protein.

• References:

  Bredt D.S., Nature 351:714-718(1991). Magee T., Biochem. Biophys. Res. Commun. 226:145-151(1996). Uvarov V.Y., Biochem. Biophys. Res. Commun. 206:736-741(1995).

Peptide - + Western blot analysis of extracts from RAW264.7 cells, treated with INF (2500u/ml, 10mins), using n-NOS (Ab-852) antibody. Immunofluorescence analysis of HeLa cells, using n-NOS (Ab-852) antibody.