Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human NF-kB p65 around the phosphorylation site of threonine505 (L-V-TP-G-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
NF-κB p65 (Ab-505) antibody detects endogenous levels of total NF-κB p65 protein.
References:
Davide Ferrari, J. Cell Biol., Dec 1997; 139: 1635.
Ivan Mattioli, J. Immunol., May 2004; 172: 6336 - 6344.
Susumu Tomita, J. Biol. Chem., Apr 2000; 275: 13056.
Mihaela Gadjeva, J. Immunol., Nov 2004; 173: 5786 - 5793.
Western blot analysis of extracts from HeLa cells, using NF-κB p65 (Ab-505) antibody.