• Catalog Number:

  E1A0512B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human MITF around the phosphorylation site of serine 180/73 (P-N-SP-P-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O75030

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  MITF M1, Microphthalmia-associated transcription factor

• Specificity Sensitivity:

  MITF (Ab-180/73) antibody detects endogenous levels of total MITF protein.

• References:

  Jón H. Hallsson, Genetics, May 2004; 167: 233 - 241. Eiichi Morii, Blood, Apr 2001; 97: 2038 - 2044. Eiichi Morii, Blood, Sep 2004; 104: 1656 - 1661.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human skin tissue using MITF (Ab-180/73) antibody. Western blot analysis of extracts from HepG2 cells (lane 1) and COLO205 cells (lane 2), using MITF (Ab-180/73) antibody.

Peptide - + Immunofluorescence analysis of HeLa cells, using MITF (Ab-180/73) antibody.