• Catalog Number:

  E1A0508B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human MDM2 around the phosphorylation site of serine 166 (A-I-SP-E-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q00987

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Double minute 2 protein, EC 6.3.2.-, Oncoprotein Mdm2, Ubiquitin-protein ligase E3 Mdm2, p53-binding protein Mdm2

• Specificity Sensitivity:

  MDM2 (Ab-166) antibody detects endogenous levels of total MDM2 protein.

• References:

  M Zhou, Blood, Mar 1995; 85: 1608 - 1614. Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030. M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228. Jordan S. Fridman, Cancer Res., Sep 2003; 63: 5703 - 5706.

Peptide - + Western blot analysis of extracts from COS7 cells, using MDM2 (Ab-166) antibody. Immunofluorescence analysis of NIH/3T3 cells, using MDM2 (Ab-166) antibody.