Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human JNK1/2/3 around the phosphorylation site of threonine 183/tyrosine 185 (M-M-TP-P-YP-V-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
JNK1/2/3 (Ab-183/185) antibody detects endogenous levels of total JNK1/2/3 protein.
References:
Robert A. Kaiser, J. Biol. Chem., Sep 2005; 280: 32602 - 32608.
Eleanor T. Coffey, J. Neurosci., Jun 2002; 22: 4335.
Nanyue Chen, Cancer Res., Mar 2002; 62: 1300 - 1304.
Nan-Shan Chang, J. Biol. Chem., Mar 2003; 278: 9195 - 9202.
Peptide - +
Western blot analysis of extracts from 293 cells, treated with UV (5mins), using JNK1/2/3 (Ab-183/185) antibody.
Immunofluorescence analysis of HeLa cells, using JNK1/2/3 (Ab-183/185) antibody.