Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CREB around the phosphorylation site of serine 121 (T-D-SP-Q-K).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P16220
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
CREB-1, CREB1, cAMP-response element binding protein
Specificity Sensitivity:
CREB (Ab-121) antibody detects endogenous levels of total CREB protein.
References:
Lionel Groussin, J. Clin. Endocrinol. Metab., Jan 2000; 85: 345 - 354.
Deepa B. Shankar, Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 367.
LA Berkowitz and MZ Gilman, PNAS, Jul 1990; 87: 5258.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using CREB (Ab-121) antibody.
Western blot analysis of extracts from HeLa cells, treated with Insulin (0.01U/ml, 15mins), using CREB (Ab-121) antibody.