Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CD3 ζ around the phosphorylation site of tyrosine 42 (G-L-YP-Q-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
CD3 ζ (Ab-42) antibody detects endogenous levels of total CD3 ζ protein.
References:
Linda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029.
Sandeep Krishnan, J. Immunol., Sep 2005; 175: 3417 - 3423.
Linda A. Trimble, J. Virol., Aug 2000; 74: 7320 - 7330.
Hisashi Arase, J. Immunol., Jan 2001; 166: 21.
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using CD3 ζ (Ab-42) antibody.