Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 674(A-I-YP-S-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P22681
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
CBL E3 ubiquitin protein ligase, CBL2, EC 6.3.2.-, Proto-oncogene c-CBL, Signal transduction protein CBL
Specificity Sensitivity:
CBL (Ab-674) antibody detects endogenous levels of total CBL protein.
References:
Stefanie Loeser, J. Exp. Med., Apr 2007; 204: 879 - 891.
A Oda, K Ozaki, Blood, Aug 1996; 88: 1330 - 1338.
Yajun Yi, J. Immunol., Dec 2000; 165: 6627.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CBL (Ab-674) antibody.
Western blot analysis of extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), using CBL (Ab-674) antibody.