• Catalog Number:

  E1A0464B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 774(D-G-YP-D-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P22681

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  CBL E3 ubiquitin protein ligase, CBL2, EC 6.3.2.-, Proto-oncogene c-CBL, Signal transduction protein CBL

• Specificity Sensitivity:

  CBL (Ab-774) antibody detects endogenous levels of total CBL protein.

• References:

  Stefanie Loeser, J. Exp. Med., Apr 2007; 204: 879 - 891. A Oda, K Ozaki, Blood, Aug 1996; 88: 1330 - 1338. Yajun Yi, J. Immunol., Dec 2000; 165: 6627.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CBL (Ab-774) antibody. Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using CBL (Ab-774) antibody.

Peptide - + Immunofluorescence analysis of A549 cells, using CBL (Ab-774) antibody.