• Catalog Number:

  E1A0459B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from humanAurora Kinase around the phosphorylation site of threonine 288 (R-T-TP-L-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14965

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  AIK, ARK1, AYK1, Aurora-A, Aurora-family kinase 1, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, BTAK, Breast-tumor-amplified kinase, EC 2.7.11.1, IAK1, Ipl1- and aurora-related kinase 1, STK15, STK6, Serine/threonine kinase 15, Serine/threonine kinase 6, Serine/threonine kinase Ayk1, kinase AurA,AurA

• Specificity Sensitivity:

  Aurora Kinase (Ab-288) antibody detects endogenous levels of total Aurora Kinase protein.

• References:

  Kimura M., J. Biol. Chem. 272:13766-13771(1997). Shindo M., Biochem. Biophys. Res. Commun. 244:285-292(1998). Deloukas P., Nature 414:865-871(2001). Sasayama T., Genes Cells 10:627-638(2005).

Western blot analysis of extracts from 293 cells, treated with serum (20%, 15mins), using Aurora Kinase (Ab-288) antibody.