Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Ku70 around the phosphorylation site of serine 5(W-E-SP-Y-Y).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P12956
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
70 kDa subunit of Ku antigen, ATP-dependent DNA helicase II, 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC75, CTCBF, G22P1, Lupus Ku autoantigen protein p70, TLAA, Thyroid-lupus autoantigen
Specificity Sensitivity:
Ku70 (Ab-5) antibody detects endogenous levels of total Ku70 protein.
References:
Nicholas S. Y. Ting, Nucleic Acids Res., Apr 2005; 33: 2090 - 2098.
Suparna Mazumder, Mol. Cell. Biol., May 2007; 27: 3511 - 3520.
Roopashree Narasimhaiah, Cereb Cortex, Jun 2005; 15: 696 - 707.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Ku70 (Ab-5) Antibody.
Western blot analysis of extracts from HeLa cells, using Ku70 (Ab-5) Antibody.