Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3 around the phosphorylation site of threonine 3 (A-R-TP-K-Q).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%);
Rat (Identities = 100%, Positives = 100%)
Histone H3 (Ab-3) antibody detects endogenous levels of total Histone H3 protein.
References:
Zhong R., Nucleic Acids Res. 11:7409-7425(1983).
Marashi F., Biochem. Cell Biol. 64:277-289(1986).
Albig W., Genomics 10:940-948(1991).
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H3 (Ab-3) antibody.
Western blot analysis of extracts from A549 cells, using Histone H3 (Ab-3) antibody.